BD CELLine flasks

Nanci Donacki nancielaine at earthlink.net
Wed Oct 3 16:30:24 EST 2001


If you mean the ones made by Integra and sold by BD, then yes.  I use them
all the time for producing antibodies.  We use the CL350's most often, and
have great success with them.  We've modified the instructions a bit, in
terms of harvest , re-inoculation and replacing the nutrient medium and they
work well for us.  Most of our runs are for 6 weeks, but we've kept some
around for as long as 4 months of continuous production.
We've only encounted two problems in the three years that we've used them.
In one the cap liner fell out, but we now keep extra caps on hard.  One
other seemed to "spring a leak" one weekend.  It wasn't contaminated, so I
don't really know why that happened.
If you have any other questions, please contact me directly.

-ned

--
Nanci E Donacki
Research Manager, Biochemistry
MedImmune, Inc.
35 W. Watkins Mill Road
Gaithersburg, MD 20878
Phone:  (301)527-4575
Fax:  (301)527-4200
e-mail:  donackin at medimmune.com

"Paula Lavery" <Paula.Lavery at McGill.ca> wrote in message
news:Y41u7.24902$JX2.2714576 at carnaval.risq.qc.ca...
> Has anyone tried using BD's new CELLine flasks? I'd like to hear about any
> experiences with this product, good or bad.
>
> Thanks!
>
>
> --
> Paula Lavery
> Transplant Immunology Lab.
> Royal Victoria Hospital
> McGill University
> Montreal, QC, Canada
>
> "If we knew what we were doing, it wouldn't be
> called research, would it?" - Albert Einstein
>
>
>
>
>
>





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