in vitro differentiation of mouse nk cells

Dr. Hannes Klump klump at
Mon Oct 8 08:08:38 EST 2001

Hi folks!
I would like to differentiate bone marrow of C57Bl/6 mice into NK-cells
in vitro. I am currently keeping the BM cells in IMDM, 15%FCS, SCF, IL3
To which cytokines & concentrations do I have to switch? Which
publication desribes the necessary conditions?
Many thanks in advance!


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