RNA from Monocytes

sonja sonja.steppan at uni-konstanz.de
Fri Oct 19 17:59:42 EST 2001


Does anybody out there have experience how to prepare total RNA from
Monocytes?
  I wanted to prepare total RNA from stimulated Monocytes (800 000
cells) after 24h incubation in RPMI media and have used Qiagen RNeasy
kit- but with very bad yields. Any advice about Monocytes in culture
and RNA preps from Monocytes is highly appreciated. Thanks in advance.
                    Sonja




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