RNA from Monocytes

Roshni Mitra roshni at ccmb.ap.nic.in
Sat Oct 20 08:55:08 EST 2001


Dear Sonja,
it is very difficult to extract RNA from monocytes and macrophages due to
low yield.... It is not possible to see the RNA pellet with such few
cells... I recommend that you take at least 5 million cells to start with
in order to get a decent amount of RNA for your subsequent work... I use
Trizol method with good results on rat macrophages.... make sure you wash
the medium off carefully.
Roshni

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