antibody storage!!

Tim none at none.com
Fri Apr 26 13:09:09 EST 2002


Hello Dinakara,

Yes, bacterial contamination can contribute to an increase in pH.  You might
have a microbiology lab try to grow a bug out the serum but it's probably
more work than it's worth (unless you want some "peace of mind" in knowing
that you might need to be more careful with your sterile technique in the
future).

Tim

"Dinakara R.Ampasala" <ampasaladr at yahoo.com> wrote in message
news:20020426172949.5095.qmail at ww02.jatek.com...
> Hi!
> I am Dinakar working as Post doctoral fellow at Great Lakes Forestry
Centre
> in Sault Ste. Marie, Canada. I got a  little problem in antibody storage,
> recently ( four months back) I have receievd some anti body vials
(10-15ml,
> ploy clonal rabit serum) for our purified protien from one local company,
> and I stored them at -20, they worked very well when I received.
>
>       Last week I used for hybridization, none of them working and I
shocked
> to notice its pH at about14.
>
>           I failed to understand howcome its pH raised to 14? is there any
> possiblity to chenage its pH by contamination or is there any possiblity
to
> another person involvement in that ( excuse me). I am totaly lost, because
> when I checked with company people they told antibody pH should be between
> 7-8.
>
>          Please help me to understand how come its pH changed to 14.
>
> Looking forward to hear from you.
> With warm regards!
> Dinakara R. Ampasala.
>
>
> <http://www.biowww.net/forum/read.php?f=6&i=1001&t=1001>
>





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