Myeloperoxidase Assay

Ants J. anthonyjj at blueyonder.co.uk
Fri Apr 26 15:48:42 EST 2002


try contacting the Protein Reference Unit at St Georges Hospital Medical School
in London. They do it almost daily and are very helpful.

""David L Haviland"" <David.L.Haviland at uth.tmc.edu> wrote in message
news:00bc01c1ed40$dee00e00$3ca46a81 at uth.tmc.edu...
Greetings:

Has anyone out there in net-land ever done the MPO assay?   I'm using as a
means to assess (ideally) neutrophil recruitment to areas of inflammation.
The (HTAB, O-Dianisidine HCl) assay is working very well but I'm having some
trouble in converting the slope, change in OD/time, into MPO units.   I'd
appreciate any assistance with this aspect of the protocol.

Thanks in advance,
David
=============================
 David L. Haviland, Ph.D.
 Asst. Prof. Immunology
 University of Texas - Houston, H.S.C.
 Institute of Molecular Medicine
 2121 W. Holcombe Blvd.
 Houston, TX  77030
 Internet:"david.l.haviland at uth.tmc.edu"
 Voice: 713.500.2413  FAX: 713.500.2424
 ------------------------------------------------------
Obstacles are those frightful things you see when you
take your eyes off your goal.    -Hannah More
=============================

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