phagocytosis and killing assay for macrohages

Mark Haynes mark.haynes at mail.tju.edu
Mon Apr 29 09:53:34 EST 2002


"Virupakshagouda Patil" <veeru at biochem.iisc.ernet.in> wrote in message
news:20020429072331.26900.qmail at ww02.jatek.com...
> hello,
>
> I am working with mouse peritoneal macrophages..
>  1.can anybody please suggest a simple quantitative microplate based
> phagocytosis and bactericidal assay (preferably non FACS based )?
> 2. How to take out adherent macrophages from TC plastic ware without much
> damage?
>
> thanks in advance...
>
> veeru

I don't know about the first question you asked but for getting Macs off
plates you could try
a) cold media
b) versene, which is essentially a buffered EDTA solution
PEACE





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