Hello I am interested in using a glycoprotein as an antigen in human PBMC cultures. The protien is available commercially and its buffer composition is Tris-HCl, NaCl, 0.1% Triton X-100, CaCl2, Sodium Azide (.05%) and 50% Glycerol, pH 7.5. How should I treat this protein to make it fit for the culture use. N MISRA ---