Antigen prep

Namita2702 at Namita2702 at
Thu Aug 1 08:07:02 EST 2002

I am interested in using a glycoprotein as an antigen in human PBMC cultures. The 
protien is available commercially and its buffer composition is Tris-HCl, NaCl, 
0.1% Triton X-100, CaCl2, Sodium Azide (.05%) and 50% Glycerol, pH 7.5.
How should I treat this protein to make it fit for the culture use.


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