FACS staining of macrophages and quantitation of apoptosis in macrophages

veeru at biochem.iisc.ernet.in veeru at biochem.iisc.ernet.in
Tue Dec 24 04:52:58 EST 2002


Hi,

I am working with peritoneal macrophages.

I am currently facing following problems.

1. Nonspecific staining in case of macrophages. Right now I am using 2% normal mouse serum and rat serum for blocking but still the staining is not proper.

COULD YOU PLEASE LET ME KNOW....EFFECTIVE BLOCKING METHODS FOR MACROPHAGES?....  

2. What are  the effective methods for the quantification of apoptosis in case of macrophages?

thanks in advance...
bye
veeru

http://biowww.net/mynews/tree.php?group_name=bionet_immunology&begin=0



More information about the Immuno mailing list