FACS staining of macrophages and quantitation of apoptosis in macrophages

[veeru at biochem.iisc.ernet.in]

Hi,

I am working with peritoneal macrophages.

I am currently facing following problems.

1. Nonspecific staining in case of macrophages. Right now I am using 2% normal mouse serum and rat serum for blocking but still the staining is not proper.

COULD YOU PLEASE LET ME KNOW....EFFECTIVE BLOCKING METHODS FOR MACROPHAGES?....  

2. What are  the effective methods for the quantification of apoptosis in case of macrophages?

thanks in advance...
bye
veeru

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