Double Radial Immunodiffusion

vetimmunol at hau.nic.in vetimmunol at hau.nic.in
Mon Feb 18 00:59:44 EST 2002


Dear Andy,

Here are some of the possible uses of DID and IEP:
A. DID:
1. Comparing different antigens in a solution having mixture of 
antigens.
2. To check specificity of the antisera.
B. SRID:
1. Quantitation of the antigen.
C. IEP:
1. To resolve serum proteins.
2. To check monospecificity of the antisera.
Disadvantage:NEEDS LARGE AMOUNT OF SERA.

YOU CAN SEE THE FOLLOWING REFERENCES:
1.Oudin,J.(1980). Immunochemical analysis by antigen-antibody 
precipitation in gels,in Meth.Enzymol.,Vol.70,AP,166-198.
2. Ochterlony,O. and Nilson,L.A.(1986). Immunodiffusion and 
Immunelectrophoresis, in Handbook of experimental immunology,Vol 1
(Weir,D.M. et al.),Blackwell,Oxford,pp 32.1-32.5  

Good Luck!

Devender Kr. Saini,
Immunodiagnosis Lab,
Dept. Veterinary Parasitology,
College of Veterinary Sciences,
CCS Haryana Agricultural University,Hisar,Haryana,
India-125004.


----- Original Message -----
From: Andy <odonnell975 at btinternet.com>
Date: Sunday, February 17, 2002 4:20 am
Subject: Double Radial Immunodiffusion

> Hi, I was wondering if anyone could tell me of some possible 
> practical uses
> for immunoelectrophoresis and double radial immunodiffusion? I 
> know it these
> are used to study antibody-antigen interactions, but could anyone 
> give a
> possible example? Any help would be greatly appreciated.
> 
> Andy
> 
> 
> 

---




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