IFN ELIspot

Simon Frost frostie.football at btinternet.com
Thu Jan 24 14:58:21 EST 2002


Hi

I am using an IFN ELIspot assay to detect CTL activity against self
antigens. The CTLs are primary human cells and have been through three
rounds of stimulation . However, the signal to noise:ratio is very high. I
have tried optimising the E:T ratio but this has not alleviated the problem.
Can anyone suggest a way round these problems.


Thankyou in advance


Frostie





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