T2 cell lines leaky

Ian A. York iayork at panix.com
Sat Mar 30 18:33:44 EST 2002

In article <3CA63F76.9990DF1 at mdacc.tmc.edu>,
shahram mori  <smori at mdacc.tmc.edu> wrote:
>has anyone had problems with T2- cell lines being leaky.. we get 50%
>non-specific killing of our targets despite the fact that we use T-cell

(1) Have you tested for mycoplasma?

(2) If your T cell lines have NK or LAK-like activity, T2 would probably 
be a good target, being MHC low.

    Ian York   (iayork at panix.com)  <http://www.panix.com/~iayork/>
    "-but as he was a York, I am rather inclined to suppose him a
     very respectable Man." -Jane Austen, The History of England

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