BALF sampling..please help

mat b mat at ruggspotting.hn.org
Sun May 5 18:52:14 EST 2002


I am collecting BALF samples from mice with the intent of measuring IgG
levels. I was wondering if there is a way to prove that the lung has not
been damaged during sampling as i am interested in IgG that has crossed the
epithelia, and contamination with IgG from the underlying tissue/and vessels
would invalidate results. Samples with haemaglobin are being discarded, but
this is not satisfactory.
If anyone could help i woul be very grateful
cheers


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