Making antiserum for WB and IP

dustjohn at dustjohn at
Sat Nov 2 16:33:54 EST 2002

What is the best method for producing rabbit polyclonal antiserum that can both IP the protein of interest and recognize it by WB?
Is it best to inject separate rabbits, one with denatured antigen and the other with native antigen?  Or can one inject the same rabbit with different antigen preps?  

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