IgA-producing hybridomas

Mike Clark mrc7 at cam.ac.uk
Tue Oct 1 06:19:08 EST 2002


In article <e794ccc8.0209302309.159110f5 at posting.google.com>, Daniele Focosi
<URL:mailto:mi at interhealth.info> wrote:
> Do anyone know if strategies to trigger in vitro class switch
> recombination towards IgA production exist ? If not, do other
> strategies for creating IgA-producing hybridomas exist apart from
> fusing with a IgA-producing plasma cell ? I refer just to monomeric
> IgA, so I'm not interested in secretory component.
> Many thanks!
> 
> Daniele
> 
You could of course clone the V-gene and then express it as a recombinant
IgA.

Bruggemann,M., Williams,G.T., Bindon,C.I., Clark,M.R., Walker,M.R.,
  Jefferis,R., Waldmann,H., & Neuberger,M.S. (1987), J. Exp. Med. 166,
  1351-1361  Comparison of the effector functions of human immunoglobulins
  using a matched set of chimeric antibodies.

Bruggemann,M., Teale,C., Bindon,C., Clark,M., & Waldmann,H. (1989), J.
  Immunol. 142, 3145-3150  A matched set of rat/mouse chimeric antibodies.

Dunne,D.W., Richardson,B.A., Jones,F.M., Clark,M., Thorne,K.J.I. &
  Butterworth,A.E. (1993), Parasite Immunology 15, 181-185  The use of
  mouse/human chimaeric antibodies to investigate the roles of different
  antibody isotypes, including IgA2, in the killing of Schistosoma mansoni
  schistosomula by eosinophils

The above papers refer to human and rat IgA but the same principles apply
to recombinant expression of IgA from any other species.

Mike                            <URL:http://www.path.cam.ac.uk/~mrc7/>
-- 
 o/ \\    //          ||  ,_ o   M.R. Clark, PhD. Division of Immunology
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