Immunoprecipitation...

FreD f.boal at iecb-polytechnique.u-bordeaux.fr
Fri Oct 25 02:35:24 EST 2002


thank you, it is a good idea! very simple to use!

Allison <"allison.haggartyREMOVE THIS"@mcgill.ca> wrote in message news:<3DB6AAEE.30F5059C at mcgill.ca>...
> FreD wrote:
> 
> > Hi all!
> >
> > i am a phd student (bordeaux, France;) and i have some problems with
> > an IP. My protein is about 30kDa, and the dimer is about 60kDa. So i
> > have some problems to see them on SDS-PAGE (thanks light and heavy IgG
> > chains!).
> 
> What if you run a gel without reducing agent.  Then the intact antibody
> would run at 150K leaving your 30K and 60K bands on their own.
> 
> Allison
> 
> 
>



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