Tris & PAGE

[Andrew Hall]

Hi all,

Can anyone define Tris for me? I have spent all day researching this
attempting to piece together some sort of unifying concept of Tris to no
avail. In our (older) copy of Maniatis there is a section that advises the
use of Tris base, and not Trisma or Tris-HCl to make up all buffers for PAGE
since additional salts will affect the separation of proteins on the gel.
Now to make things more complicated it then refers to Tris-Cl as one of the
components of such a buffer. I know that Sigma produce Trisma but is this
Tris or a derivative of? Therefore can anyone define each of these
solutions/compounds for me:

Tris (a formula for this would be useful)
Tris-HCl
Tris-Cl
Trisma

Thanks,

Andrew Hall
andrew.hall at virgin.net