Mouse monoclonal antibody

Dan Marquez dmarquez3 at socal.rr.com
Wed Sep 18 21:41:28 EST 2002


"Nanci E Donacki" <captainnanci at earthlink.net> wrote in message
news:XYNf9.143$Le2.19176 at newsread2.prod.itd.earthlink.net...
> There is no reason why you can't attach the peptide to a protein carrier
> such as KLH or ovalbumin to increase the immunogenicity.  Yes, you will
get
> antibodies to the protein as well, but you need to make sure your
screening
> assays are specific for the peptide, you should be okay.
> Another suggestion, try hamsters instead of rats.  DNA is also an option,
> although the gene gun approach seems to be more successful than im
> injections of DNA.
> -ned
>
> "Daniele Focosi" <mi at interhealth.info> wrote in message
> news:e794ccc8.0209111128.5ddb060b at posting.google.com...
> > I would like to suggest you to prove DNA immunization for better
> > results. Anyway please note I'm just a PhD student and I have not real
> > experience with such a technique apart from literature results !
> > Please let me know.
> > Best regards.
> >
> > Daniele
> >
> > "news.dk.uu.net" <myd at nordicbioscience.com> wrote in message
> news:<3d78af5a$0$10685$4d4eb98e at news.dk.uu.net>...
> > > Dear all,
> > >
> > > I would like to know if it is possible to raise a mouse monoclonal
> antibody
> > > against a protein/peptide of rat. The thing is that this protein is
very
> > > well conserved among rodent and that immunizing a mouse with this
> peptide
> > > will not give much result.
> > > Does anybody know what I can do? I thought about attaching the peptide
> to a
> > > highly antigenic carrier protein but then I will get an antibody
against
> the
> > > carrier and not the peptide....Any suggestions will be very helpful.
> > >
> > > Thanks in advance
> >
>
>

Can dendritic cells be fed the protein in vitro, and then injected back with
TNF in vivo so they can present the protein to Th cells?

Dan






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