Hello,
I have one big problem to share with you guys. I did the experiment to identify Cry4B toxin binding proteins in mosquito larvae by western blotting. Well, I found that the anti-Cry4B polyclonal antibody cross-reacted with brush border membrane proteins (BBMP). In my opinion, I think the proteins cross-reacted with the antibody after they were digested by endogenous proteases. Do you think it is the good explanation. I want to hear comments from you guys.
Thank you so much. ;)
http://biowww.net/mynews/tree.php?group_name=bionet_immunology&begin=0
