Elisa blocking agent

obi_1_ at hotmail.com obi_1_ at hotmail.com
Fri Apr 18 19:01:08 EST 2003


I am doing an Elisa. I get my first protein to bing to the tray then i block then i add my second protein. However i cannot get the blocking step to work. Ive used BSA, skim milk, tryptone peptone, egg yolk, various cell lysates. Has anyone got an good blocking agents thats relatively cheap and available?
Thanks


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