Elisa blocking agent

[Text NTL News]

Do you need to block? Have you tried a more dilute antibody?

Pete

<obi_1_ at hotmail.com> wrote in message
news:20030419000108.12976.qmail at ww02.hostica.com...
> I am doing an Elisa. I get my first protein to bing to the tray then i
block then i add my second protein. However i cannot get the blocking step
to work. Ive used BSA, skim milk, tryptone peptone, egg yolk, various cell
lysates. Has anyone got an good blocking agents thats relatively cheap and
available?
> Thanks
>
>
> http://biowww.net/mynews/tree.php?group_name=bionet_immunology&begin=0