Elisa blocking agent

Text NTL News p.crilly at ntlworld.com
Mon Apr 21 08:27:40 EST 2003

Do you need to block? Have you tried a more dilute antibody?


<obi_1_ at hotmail.com> wrote in message
news:20030419000108.12976.qmail at ww02.hostica.com...
> I am doing an Elisa. I get my first protein to bing to the tray then i
block then i add my second protein. However i cannot get the blocking step
to work. Ive used BSA, skim milk, tryptone peptone, egg yolk, various cell
lysates. Has anyone got an good blocking agents thats relatively cheap and
> Thanks
> http://biowww.net/mynews/tree.php?group_name=bionet_immunology&begin=0

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