Elisa blocking agent

Simon Worrall s.worrall at uq.edu.au
Wed Apr 23 00:51:34 EST 2003

I have used casein for many years with good effect.  You need to make a 
saturated solution (2% w/v) which is a bit of a fiddle but that will 
keep for several weeks.  You can then try different dilutions to find 
which works best (normally between 0.5 and 1% casein).  I also use it in 
all washes and in the secondary antibody   step.  You could try TWEEN 
detergent but some studies say that it can alter the immunogenicity of 
some proteins.

Simon Worrall Ph.D.
Dept of Biochemistry and Molecular Biology
University of Queensland

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