Elisa blocking agent

Damian Bond damian.bond at btconnect.com
Sun Apr 27 07:02:55 EST 2003


There are some very useful technical bulletins on the Nunc web site,
concerning use of blocking agents and detergents.
<obi_1_ at hotmail.com> wrote in message
news:20030419000108.12976.qmail at ww02.hostica.com...
> I am doing an Elisa. I get my first protein to bing to the tray then i
block then i add my second protein. However i cannot get the blocking step
to work. Ive used BSA, skim milk, tryptone peptone, egg yolk, various cell
lysates. Has anyone got an good blocking agents thats relatively cheap and
available?
> Thanks
>
>
> http://biowww.net/mynews/tree.php?group_name=bionet_immunology&begin=0





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