I wonder if anyone could shed some light on the following question for me:
I'm interested in cloning the complete cDNA of IgE secreted by the U266 cell line, but don't know how to get started. Should I start by constructing the entire cDNA libraries from U266? Then, where can I find the primers needed for 'fishing' out that IgE cDNA I need?
Any suggestion will be highly appreciated, thanks.