U266 cDNA libraries cloning

bhchen at hsc.vuc.edu bhchen at hsc.vuc.edu
Tue Apr 29 00:19:07 EST 2003


Hi, all:

I wonder if anyone could shed some light on the following question for me:

I'm interested in cloning the complete cDNA of IgE secreted by the U266 cell line, but don't know how to get started.  Should I start by constructing the entire cDNA libraries from U266?  Then, where can I find the primers needed for 'fishing' out that IgE cDNA I need?  

Any suggestion will be highly appreciated, thanks.

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