Establishing stabel cell lines

Geronimo user at server.com
Sat Aug 23 02:48:52 EST 2003


Make sure the concentration that kills the cells does kill them all (or at
least inhibits their growth). Then just increase the selection concentration
twofold and you should be able to distinguish positive transfectants from
controls.

<shannon.norland at stjude.org> wrote in message
news:20030822155629.17990.qmail at ww02.hostica.com...
>
> We are trying to establish two stable cell lines using G418 resistance and
hygromycin resistance.  After determining the concentration of each drug
needed to kill the cells, we proceeded with transfections.  Some of the
control cells (approx. 30%), those transfected with no DNA, did not die.
Has anyone encountered this problem before?  If so, what actions did you
take?  Could you still establish a stable cell line?
>
> Thanks.
>
> http://biowww.net/mynews/tree.php?group_name=bionet_immunology&begin=0





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