biotinylated protein for amplification

Tony O'Connor tony.oconnor at may.ie
Wed Feb 5 09:33:17 EST 2003


I want to biotinylate a protein to use as an "amplification layer" in
an ELISA, as follows:

[Solid-phase]-(Ab)-(Ag)-(biotinylated Ab)-(streptavidin)-
*{biotinylated protein}n*-(HRP-streptavidin)n

I'm doing this to replace a biotinylated anti-streptavidin Ab which is
not amplifying the signal. I'm going to try BSA - there's always BSA
in the lab :-)
Has anyone tried other proteins (adequately-sized with several
non-adjacent accessible amino groups) in such a system?

Thanks,
Tony

Tony O'Connor
Institute of Immunology
NUI Maynooth
Ireland
tony.oconnor at may.ie




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