Dendritic Cells and Autofluorescence

Mr. Hat misterhat at sbcglobal.net
Mon Jul 7 01:05:28 EST 2003


In article <20030630162751.26204.qmail at ww02.hostica.com>,
skassi at lsuhsc.edu wrote:

> I'm having problems with high levels of autofluroescence for my
BM-derived cultured dendritic cells.  Any suggestions for how I can reduce
and/or eliminate the autofluorescence?
> 
> http://biowww.net/mynews/tree.php?group_name=bionet_immunology&begin=0

I'm assuming you're doing FACS analysis. WHat machine are you using? WHat
channels (or specifically dyes) are you using that you are seeing
interfered with?


M.H.



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