TMB substrates

mclellan12xu at mclellan12xu at
Thu Oct 23 20:33:46 EST 2003

This recipe works very well:

8.2g sodium acetate anhydrous
3.6g citric acid 
dissolve in 600ml water then add: 
270mg TMB (from Fluka) dissolved in 400ml methanol
store in dark at 4oC. 

Add 5 micro litres of 30% hydrogen peroxide per 10ml TMB substrate immediately prior to use. Stop with 50microlitres 2N sulphuric acid.

Sorry I can't provide an origin or reference for this recipe.

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