TMB substrates

Nanci Donacki captainnanci at earthlink.net
Fri Oct 24 20:35:57 EST 2003


Why not just buy the pre-made 1 component TMB from KPL?

<mclellan12xu at yahoo.com> wrote in message
news:20031024013346.7709.qmail at ww02.hostica.com...
> This recipe works very well:
>
> 8.2g sodium acetate anhydrous
> 3.6g citric acid
> dissolve in 600ml water then add:
> 270mg TMB (from Fluka) dissolved in 400ml methanol
> store in dark at 4oC.
>
> Add 5 micro litres of 30% hydrogen peroxide per 10ml TMB substrate
immediately prior to use. Stop with 50microlitres 2N sulphuric acid.
>
> Sorry I can't provide an origin or reference for this recipe.
>
>
>
>
>
> http://biowww.net/mynews/tree.php?group_name=bionet_immunology&begin=0





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