culturing OKT8

Venkatesh Prasanna KS venky at jncasr.ac.in
Fri Sep 12 06:38:26 EST 2003


Hello World,
I am a new-comer to the world of hybridoma technology. I am culturing OKT8 
cells and am having problems in amplifying them. Though they are doing 
good in 100mm plates,but  the same is not true in 150mm plates. I have 
tried plates from different companies to eliminate the remote  possibility of 
plastic toxicity. I am using 10% DMEM. 

If anybody has any suggestions as to what the problem could be please do 
let me.

Thanks
Venkatesh   

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