T cell activation by Staphylococcal enterotoxin B

Becky Kelly eak at medicine.wisc.edu
Fri Apr 2 14:33:28 EST 2004


Can anyone tell me if circulating human CD4+ T cells can be directly
(absence of APCs)activated by SEB?  Human blood CD4+ cells were sorted
by FACS to >98% puriety.  Cells were stimulated for 6 days with SEB (3
micrograms/ml)and sups analyzed by ELISA for IL-13 and IFN-gamma. Very
high cytokines levels were seen (100 ng/ml IFN-g and 5 ng/ml IL-13).
Any thoughts on mechanism?  Does SEB bind to a TLR?
Thanks-Becky



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