ELISA Problem

[Jason Bubier]

Hello,
    I am doing an ELISA assay for a cytokine in the serum of mouse. My 
standard curve looks great (4000-63 pg/ml) but it is undetectable in all 
serum samples EVEN when I spike the sample with 250 pg/ml of cytokine which 
works fine in the standard curve! Colleagues have suggested this is due to 
interfering factors from the serum. I have performed dilutions of 1:10 and 
1:4 but still no detection. Any advice would be greatly appreciated as to 
what parameters I should vary. I was referred to "The Immuno Assay" by 
David Wild which I don't have but am ordering. If you have the book let me 
know what it says about controlling interfering substances.

Thank You
Jason Bubier

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