ELISA PROBLEM Details

Jason Bubier jbubier at jax.org
Mon Aug 16 10:25:36 EST 2004


What kind of ELISA (direct, indirect, sandwich)? SANDWICH	
-What cytokine is of interest? IL-15 FAMILY		
-Are you using a commercially available antibody, or 
home-brewed?  COMMERCIAL R&D Systems	
-What are you using as a blocking solution? PBS + 1% BSA
-Are you putting your antibodies in the blocking solution? CAPTURE is in 
PBS, detection is diluted in blocking solution

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