ELISA problem

Maria Gacis MariaG3 at chw.edu.au
Mon Feb 2 22:44:42 EST 2004

We are using a robotic pipettor to dispense ELISA reagents, and have found
introduction of a contaminant which affects colour development, increasing
the non-specific background.   Based on the pattern of non-specific colour,
we believe that the contaminant is introduced at the step in which the
horseradish peroxidase-anti-human IgG is dispensed. 

Can anyone suggest  either the source of the contaminant, its nature, or a
possible solution to the problem ? 



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