Cell-surface molecule control for FACS

Ian A. York iayork at panix.com
Wed Oct 20 14:57:25 EST 2004


I'm looking for a molecule as a control for flow cytometry.  What I need 
is a protein with the following characteristics:
 o  Expressed on many (human) tissue types and cell lines
 o  Expressed at relatively high levels, so easily detected
 o  Expressed at relatively constant levels (i.e. minimally sensitive to 
cell cycle stage or stress)
 o  Antibodies easily available
 o  NOT MHC class I, since that's what I'm trying to control for.

I'm wondering about some of the integrins, perhaps, but I'm not sure which 
are widely-enough expressed; but any protein that meets the 
characteristics above would be OK.

Ian 
-- 
    Ian York   (iayork at panix.com)  <http://www.panix.com/~iayork/>
    "-but as he was a York, I am rather inclined to suppose him a
     very respectable Man." -Jane Austen, The History of England



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