Cell-surface molecule control for FACS

Mike Clark mrc7 at cam.ac.uk
Thu Oct 21 06:30:51 EST 2004


In article <cl6fv4$j03$1 at reader1.panix.com>, Ian A. York
<URL:mailto:iayork at panix.com> wrote:
> I'm looking for a molecule as a control for flow cytometry.  What I need 
> is a protein with the following characteristics:
>  o  Expressed on many (human) tissue types and cell lines
>  o  Expressed at relatively high levels, so easily detected
>  o  Expressed at relatively constant levels (i.e. minimally sensitive to 
> cell cycle stage or stress)
>  o  Antibodies easily available
>  o  NOT MHC class I, since that's what I'm trying to control for.
> 
> I'm wondering about some of the integrins, perhaps, but I'm not sure which 
> are widely-enough expressed; but any protein that meets the 
> characteristics above would be OK.
> 
> Ian

Ian, you don't say what you mean by many human tissues? 

Clearly there are pan-leukocyte markers such as CD45 (Leukocyte Common
Antigen) or the common integrin beta subunit CD18, which will be
expressed on a large number of cell lines and blood cell types.

But other markers you might like to consider are things such as  the
complement regulatory markers such as CD59 and decay accelerating factor
(DAF) which are expressed quite widely on tissues exposed to complement
plasma factors. Both CD59 and DAF are GPI anchored if that makes a
difference to your experiments? 


Mike                             <URL:http://www.path.cam.ac.uk/~mrc7/>
-- 
M.R. Clark, PhD. Division of Immunology
Cambridge University, Dept. Pathology
Tennis Court Rd., Cambridge CB2 1QP
Tel.+44 1223 333705  Fax.+44 1223 333875




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