mouse neutrophils isolation

maboucabeille at maboucabeille at
Tue Sep 14 10:53:17 EST 2004


I try to isolate mouse neutrophils using dicontinuous Percoll gradient. I have contamination with lymphocytes and RBC. Does somebody use a protocol which works nicely and permits the isolation of a quasi pure and non activated population of mouse neutrophils?

Thanks a lot for your answer,

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