mouse neutrophils isolation

maboucabeille at access.unizh.ch maboucabeille at access.unizh.ch
Tue Sep 14 06:03:24 EST 2004


Hi,

I have some problems to isolate mouse neutrophils. I use discontinuous Percoll three steps (or two) gradient but I have contamination with lymphocytes and RBC. Does somebody have a good method to  isolate mouse neutrophils (without activating them?)

Thanks a lot,
Marie-Aude


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