[Immunology] Glycerol and stability of Immunoglobulins
KAAD (Karina Agergaard Andersen)
kaad at novonordisk.com
Wed Apr 5 04:13:24 EST 2006
I have observed a problem with a standard in one of our ELISA assays.
The standard is immunoglobulins diluted in glycerol (63%), Imidazol,
Thimerosal, HSA, Tween 20 and water. Normally the standard was kept at
-20C (in dilution), but was then moved to -80C. During a period of about
4-5 month a jump to higher responses of the standard was suddenly
observed and at the same time it was noticed that the standard solution
Can anobody tell me why a higher respons is observed? I would expect a
lower respons because of aggregation of the antibodies when the solution
is freezed. I also wonder that the lag period of 4-5 months is observed.
Any references to documentation of glycerol and its impact on stability
of immunoglobulins are very appreciated.
Thank you in anticipation
Karina Agergaard Andersen
FVII QC Lab
Novo Nordisk A/S
44 43 02 82 (direct)
KAAd at novonordisk.com
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