[Immunology] Glycerol and stability of Immunoglobulins

KAAD (Karina Agergaard Andersen) kaad at novonordisk.com
Wed Apr 5 04:13:24 EST 2006

I have observed a problem with a standard in one of our ELISA assays.
The standard is immunoglobulins diluted in glycerol (63%), Imidazol,
Thimerosal, HSA, Tween 20 and water. Normally the standard was kept at
-20C (in dilution), but was then moved to -80C. During a period of about
4-5 month a jump to higher responses of the standard was suddenly
observed and at the same time it was noticed that the standard solution
was freezed.

Can anobody tell me why a higher respons is observed? I would expect a
lower respons because of aggregation of the antibodies when the solution
is freezed. I also wonder that the lag period of 4-5 months is observed.
Any references to documentation of glycerol and its impact on stability
of immunoglobulins are very appreciated.

Thank you in anticipation


Karina Agergaard Andersen

Novo Nordisk A/S
Sauntesvej 13
DK-2820 Gentofte
44 43 02 82 (direct)
KAAd at novonordisk.com

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