[Immunology] Non radioactive Cytotoxic activity assay

Eniafe, Rhoda (NIH/NHLBI) [E] eniafer at nhlbi.nih.gov
Fri Apr 7 10:49:51 EST 2006


Hello David, 

I have been try to use Calcein-AM in Cytotoxic assay, but I am having
problem with leakage and inconsistencies with the killing assay, do you
encounter this kind of problem(s) and what are the steps you take to
prevent it.

Thanks 

Rhoda


	Rhoda Eniafe, MT(ASCP)
	Stem Cell Allogeneic Transplantation Section
	HEMATOLOGY BRANCH,NHLBI,NIH
Mail address: Bldg 10 (CRC), Rm. 3E-5288, MSC 1202
	Office address: Bldg 10 (CRC), Rm. 3E-5288
	Phone# 301-435-2624; Fax# 301-480-2664
	email: eniafer at nhlbi.nih.gov






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