[Immunology] Stimulation of monocytes for cytokine production

Zelnickova, Petra ZelnickovaP at VFU.cz
Tue Apr 25 09:12:42 EST 2006


Hello,

I am trying to detect IL-1beta, IL-6, IL-8 and TNF-alpha in peripheral blood monocytes in pig by flow (I tried to stimulate the whole blood as well as PBMCs isolated on Histopaque). I have problem to stimulate these cells for cytokine production. PMA + ionomycin works in lymphocytes only (stimulates TNF-alpha production in lymphocytes). Three different LPSs which I tested didnt work. Moreover there is relatively high spontaneous production of IL-1beta and IL-8 which is paradoxically downregulated after longer stimulation (>8 hours) with LPS or PMA + ionomycin. Can anybody help me how to stimulate monocytes for above mentioned cytokine production?

Thanks

Petra Zelnickova




Petra Zelnickova
Institute of Physiology
University of Veterinary and Pharmaceutical Sciences Brno
Palackeho 1-3
612 42 Brno
email: zelnickovap at vfu.cz


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