[Immunology] Re: blocking Fc receptors

Allison allison at nospam.com
Tue Jan 31 10:05:13 EST 2006


We do often do FACS analysis with monoclonal antibodies. As a control 
for nonspecific binding of the MAb we use an isotype matched negative 
control.

To avoid the problem of nonspecific binding of 2ndary Ab  we use 
F(ab')2  anti-IgG conjugated to FITC (or other dye).   No Fc domain 
means no binding to Fc receptors.

HTH
Allison



Sanjay Patel wrote:
> Dear Sir/Madam,
> 
>  
> 
> I'm staining mouse and human whole blood for FACS analysis. Some
> investigators have told me that I need to use Fc block for both, and some
> have said that I need Fc block only for mouse as human whole blood contains
> serum which is a natural block? What should I be using and what is the best
> block for each species?
> 
>  
> 
> Many thanks
> 
>  
> 
>  
> 
>  
> 
> Sanjay Patel
> 


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