[Immunology] ELISA for cell-seeded scaffolds

Najmuddin Gunja ngunja at gmail.com
Fri Mar 24 16:45:49 EST 2006


Hi,

I am graduate student in a tissue engineering lab and I had a question about
ELISA.

I have a cell-seeded PLLA scaffold, that is now covered with extracellular
matrix (tissue). I would like to do an ELISA test for ECM proteins, but am
not sure how to digest the scaffold without denaturing the proteins.

Does anyone have any thoughts?
Thanks!

Najmuddin


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