Hi,
Does anybody have a good protocol for activate mouse primary T-cells
from spleen? Our purpose is to study a protein which suppose to
localized in the uropods. We can see any after stimulating with
anti-CD3 and plate on FN plates. Any advice will be much appreciated.
Nian
Nian Zhang, Ph.D
Laboratory of Developmental Genetics,
Van Andel Research Institute,
333 Bostwick Ave, NE.
Grand Rapids, MI 49503.
Tel: 616 234 5312
Fax:616 234 5313
http://www.vai.org/VARI/default.asp
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