[Immunology] Immunology

Nischal Padala npadala at gmail.com
Mon May 22 15:58:58 EST 2006


Hi!

Im trying to do a mouse T cell proliferation assay. According to the
literature out there one should be able to induce CD4+ T cell proliferation
by using anti cd3 and anti cd28. But I cant seem to get this to work...i.e.
the cells are not multiplying (im measuring proliferation by using Brdu and
FACS). I have tried different concentrations for cd3 (from 3ug/ml to 25
ug/ml) and cd28 (2ug/ml to 8ug/ml). I also tried them as both platebound and
soluble antibodies. I dont know what I am doing wrong...any help/suggestions
or protocols would be greatly appreciated!

Thank You
N K


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