[Immunology] Re: Model for determination of APC funtion in vivo

Ian A. York iayork at panix.com
Thu May 25 08:22:13 EST 2006


In article <mailman.1091.1148553772.16885.immuno at net.bio.net>,
Shankar, Vijay <Vijay.Shankar at med.uni-heidelberg.de> wrote:
>
>Briefly there exists a BM1 mice which is deficient for the binding of
>SINFEHKL peptide. This mice can be used for the transplantation of APC
>from the same strain of mice to present the ovalbumin(SINFEHKL) via
>MHCII. CD8 T cell proliferation of the transplanted cell type can be
>determined using the T cells from OT1 mice. Does BM2 mice exist at all,
>bcos OT2 mice do exist.......

I'm a little puzzled here.  "SINFEHKL" is not an ovalbumin epitope and the 
sequence doesn't exist in chicken ovalbumin.  The commonly-used ovalbumin 
epitope is "SIINFEKL", but that doesn't bind to MHC class II, it binds to 
MHC class I.  ("SINFEHKL" might bind to MHC class I but is very unlikely 
to bind to class II, since it's much shorter than any MHC class I-binding 
peptide I've heard of.)  So I'm not at all sure what it is you're hoping 
to do.

OTII mice do exist, but they recognize "ISQAVHAAHAEINAGR".  

Measuring capacity of APC to present antigen is fairly straightforward, in 
general -- you can load APC in vitro with peptide, or infect them with 
virus or whatever, and then transfer them into mice and measure expansion 
of the specific T cell set you're after.

As I say I'm not quite sure what it is you want to do.

Ian 
-- 
    Ian York   (iayork at panix.com)  <http://www.panix.com/~iayork/>
    "-but as he was a York, I am rather inclined to suppose him a
     very respectable Man." -Jane Austen, The History of England


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