[Immunology] immunogenecity of prokaryotic proteins

Nausheen Jamal nausheenjamal at yahoo.ca
Wed May 31 01:24:37 EST 2006


I want to know the immunogenicity of
Glutathione-s-transferase and thioredoxin against
human serum.

I prepare recombinant human proteins, expressed with
GTS or thioredoxin tags. The protein needs to be used
as antigen for screening human serum, thus I need to
know if there will be any background
immunochemichemical reaction between the protein tags
and human serum - detectable on western blot analysis.

Any clues with resources where i can find an answer
will also be appreciated.

Thank you,


Tohoku University, Sendai, Japan.

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