[Immunology] jurkat transfection

Maria Jose De Rosa via immuno%40net.bio.net (by mjderosa At criba.edu.ar)
Fri Nov 17 11:02:18 EST 2006


Hi,
I am very interested in transfecting Jurkat Cells. I tried electroporation but the efficiency is very low. I am using a pulse of 400 V and 125 uF in electroporation buffer. Someone could say me if this is OK?
Thank you so much
Maria Jose De Rosa
Bahia Blanca
Argentina



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