[Immunology] RE: Immuno Digest, Vol 16, Issue 6

J. Diaka via immuno%40net.bio.net (by jdiaka At fau.edu)
Mon Nov 20 14:06:18 EST 2006


Hi here,

I am working with maspin (cancer suppressor gene). I am highly in need of
recombinant maspin and I am requesting that if there is anyone out there who
can give me a "little bit", I will highly appreciate it. Please I need help.

Thanks,


James




-----Original Message-----
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[mailto:immuno-bounces At oat.bio.indiana.edu] On Behalf Of
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Sent: Saturday, November 18, 2006 12:01 PM
To: immuno At magpie.bio.indiana.edu
Subject: Immuno Digest, Vol 16, Issue 6

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Today's Topics:

   1. jurkat transfection (Maria Jose De Rosa)


----------------------------------------------------------------------

Message: 1
Date: Fri, 17 Nov 2006 17:02:18 +0100
From: "Maria Jose De Rosa" <mjderosa At criba.edu.ar>
Subject: [Immunology] jurkat transfection
To: immuno At magpie.bio.indiana.edu
Message-ID: <200611171702180442.01CE5FB4 At 127.0.0.1>
Content-Type: text/plain; charset="ISO-8859-1"

Hi,
I am very interested in transfecting Jurkat Cells. I tried electroporation
but the efficiency is very low. I am using a pulse of 400 V and 125 uF in
electroporation buffer. Someone could say me if this is OK?
Thank you so much
Maria Jose De Rosa
Bahia Blanca
Argentina



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