[Immunology] Direct ELISA

Raghunath via immuno%40net.bio.net (by raghunath_kmc At yahoo.co.in)
Wed Nov 29 05:20:23 EST 2006


Hello everybody,

I am trying to standardise Direct ELISA by coating
protein as a positive control using
carbonate-bicarbonate buffer(pH 9.6),blocking with
3%BSA and then reacting with monoclonal antibody.It is
working well with standards but when i apply sample
signal/noise ratio is high and
some times negative samples are giving higher OD than
positive samples.Can u pls suggest me what could be
the reason


Thank you,
Raghunath





		
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